LOCUS       40924_43134        4608 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PCR template vector pTF104 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Fukuda T, Ohya Y, Ohta K.
  TITLE     Conditional genomic rearrangement by designed meiotic recombination 
            using VDE (PI-SceI) in yeast
  JOURNAL   Mol. Genet. Genomics 278 (4), 467-478 (2007)
  PUBMED    17587055
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Fukuda T, Ohta K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2007) Tomoyuki Fukuda, RIKEN; 2-1 Hirosawa, Wako, 
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax:81-48-462-4691)
REFERENCE   3  (bases 1 to 4608)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4608)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2007"; volume: "278"; issue: "4"; pages: "467-478"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2007) Tomoyuki Fukuda, RIKEN"; volume: " 2-1 Hirosawa, Wako,
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax"; pages: "81-48-462-4691"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..103
                     /note="sequence containing a part of the recognition
                     sequence of VDE"
     gene            complement(167..1523)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     misc_feature    1607..1707
                     /note="sequence containing a part of the recognition
                     sequence of VDE"
     promoter        complement(1737..1755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1762..1778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1920..2375
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2401..2505
                     /label=AmpR promoter
     CDS             2506..3363
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3537..4125
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4413..4434
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4449..4479
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4487..4503
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4511..4527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4548..4566
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggtacgaca tgtattccag gtgcttttgg ttgtggtaag accgttatct ctcaatcttt
       61 gtccaagtac tccaattctg acgccattat ctatgtcggg tgctttgcca agctaattcg
      121 agctcgtttt cgacactgga tggcggcgtt agtatcgaat cgacagcagt atagcgacca
      181 gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc gcatctgggc
      241 agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa aatagaacaa
      301 ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc tttttattgt
      361 cagtactgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
      421 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
      481 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
      541 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
      601 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
      661 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
      721 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac
      781 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
      841 atcaggatat tcttctaata cctggaatgc tgttttgccg gggatcgcag tggtgagtaa
      901 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
      961 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     1021 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     1081 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     1141 taatcgcggc ctcgaaacgt gagtcttttc cttacccatg gttgtttatg ttcggatgtg
     1201 atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt
     1261 ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg
     1321 tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt
     1381 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
     1441 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
     1501 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa
     1561 acagatctgg cgcgccttaa ttaacccggg gatcagcttg gagaaagtgc ggagaaagag
     1621 gtaatgaaat ggcagaagtc ttgatggaat tcccagagtt atatactgaa atgagcggta
     1681 ctaaagaacc aattatgaag cgtactactc gagggggggc ccggtaccca attcgcccta
     1741 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1801 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1861 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     1921 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1981 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2041 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2101 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2161 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2221 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2281 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2341 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     2401 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2461 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2521 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2581 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2641 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2701 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2761 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2821 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2881 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2941 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3001 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3061 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3121 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3181 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3241 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3301 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3361 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3421 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3481 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3541 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3601 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3661 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3721 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3781 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3841 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3901 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3961 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4021 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4081 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4141 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4201 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4261 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4321 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4381 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     4441 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     4501 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
     4561 taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctaga
//
